VIDEO - Membrane Proteins Workshop - The baculovirus/insect cells system with Jan H Driller
VIDEO - Membrane Proteins Workshop - The baculovirus/insect cells system with Jan H Driller
Speaker: Jan H Driller, Aarhus University, Denmark
This lecture was part of the workshop, Structural Resolution of Membrane Proteins: From Expression to Sample, organised in May 2021 by the LINXS working group Membrane Proteins, which fall under the Integrative Structural Biology theme.
Abstract
Mammalian membrane proteins are notoriously difficult to express. In contrast to bacteria or yeast, the baculovirus/insect cells system provides a good alternative to express these proteins in higher eukaryotes. Insect cells provide post-translational modifications, such as disulfide bonds or glycosylation, as well as a more akin membrane lipid composition compared to bacterial or yeast expression systems. Insect cells grow to high cell densities and using the baculovirus system even multi-protein complexes can be co-expressed.