Welcome to a seminar with Dr. Garib Murshudov from the MRC Laboratory of Molecular Biology in Cambridge, UK

Garib Murshudov is the Computational Crystallography group leader at the MRC Laboratory of Molecular Biology (http://www2.mrc-lmb.cam.ac.uk). He graduated from Baku State University, department of Applied Mathematics in 1985. Between 1987-1993, first as a visiting scientist and then as a PhD student, he worked at the Institute of Crystallography, Moscow. After defending his PhD thesis he moved to the University of York, York, UK, where he started to apply computational and statistical tools for the analysis and modelling of experimental data for structural biology. In 2001 he was awarded the Wellcome Trust Senior Research Fellowship and in 2011 he moved to the MRC-LMB, Cambridge to establish the Computational Crystallography group. His group's main interest is to develop state-of-the-art Bayesian and computational tools for building atomic and molecular models using crystallographic and electron microscopy data.

When: Friday 6th of December 2019 at 15.00-16.00
Where: Workshop room at LINXS 6th floor, Scheelevägen 19, Lund (Ideon Delta 5)

Title: Refinement of atomic models against crystallographic data and cryoEM maps

Abstract: To extract accurate and biologically relevant atomic models using noisy and limited data often produced by crystallographic experiments and cryoEM reconstructions it is necessary to design and use accurate likelihood functions linking the data and atomic models as well as a tool to utilise complementary information to the data – prior probability distributions.
In this talk a short review of the likelihood function describing the fit of atomic models into crystallographic and cryoEM data, and posterior probability distribution of “true” Fourier coefficients used for various map calculations (difference and updated maps) will be given. The remaining of the talk will focus on the design and use of prior complementary information including stereochemical information extracted from small molecular database – Crystallography Open Database, local conformational information extracted from similar macromolecular structures, information about secondary structures. Use of NMR data for crystallographic refinement will also be outlined.